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SRP6273 Sigma-Aldrich

UPA human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym: ATF, BDPLT5, PLAU, QPD, UPA, URK, Urokinase, u-PA

  •  NACRES NA.32

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Properties

biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
mol wt   calculated mol wt 45 kDa
  observed mol wt 18 kDa (DTT-reduced. Protein migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length chain due to glycosylation and cleavage. Ser21, Ile179 & Lys156 is the predicted N-terminus.)
  observed mol wt 32 kDa
  observed mol wt 50 kDa
packaging   pkg of 10 μg
  pkg of 50 μg
impurities   <1 EU/μg endotoxin (LAL test)
conjugate   6-His tagged (C-terminus)
UniProt accession no.   P00749
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... uPA(5328)

Description

General description

The PLAU (plasminogen activator, urokinase) gene is mapped to human chromosome 10q22.2.

Urokinase-type plasminogen activator is also known as PLAU and uPA. The human uPA is initially synthesized as 431 amino acid precursor with an N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule of different molecular weights. Two forms of the A chain exist, starting at Ser21 (the long form) and Lys156 (the short form). The long and short A chains are unique to the high and low molecular weight forms, respectively. The long A chain contains an EGF (epidermal growth factor)-like domain, responsible for binding of the PLAU receptor. The B chain corresponds to the catalytic domain. The gene encoding it is localized on human chromosome 10.

Biochem/physiol Actions

Urokinase-type plasminogen activator is a serine protease with extremely limited substrate specificity, cleaving the sequence Cys-Pro-Gly-Arg560-Val561-Val-Gly-Gly-Cys in plasminogen to form plasmin. It is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. uPA (plasminogen activator, urokinase) - mediated degradation of extracellular matrix of migrating cells is essential for tissue remodeling, invasiveness and angiogenesis. uPA promotes pathogenesis of Psoriasis and basal cell carcinoma. uPA activates growth factors and metalloproteinases.

Physical form

Lyophilized from 0.22 μm filtered solution in HEPES, NaCl and CaCl2. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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