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SRP6285 Sigma-Aldrich

PCSK9 human

recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE)

Synonym: FNDC5 (cleaved), Fibronectin Type III Domain-containing Protein 5 (cleaved), Fibronectin Type III Repeat-containing Protein 2 (cleaved)

  •  NACRES NA.32

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Properties

biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
mol wt   calculated mol wt 75.1 kDa
  observed mol wt 20 kDa (DTT-reduced. Protein migrates due to glycosylation and proteolytic digestion.Gln 31 is the predicted N-terminus.)
  observed mol wt 62 kDa
packaging   pkg of 20 μg
impurities   <1 EU/μg endotoxin (LAL test)
conjugate   6-His tagged (C-terminus)
UniProt accession no.   Q8NBP7
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... PCSK9(255738)

Description

General description

Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Physical form

Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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