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SRP6287 Sigma-Aldrich

LIF human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym: CDF, DIA, HILDA, LIF, MLPLI

  •  NACRES NA.32

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Properties

recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
mol wt   calculated mol wt 19.9 kDa (with Gly-Pro at the N- terminus)
  observed mol wt 35-45 kDa (DTT-reduced.Ser 23 is the predicted N-terminus .)
impurities   <1 EU/μg endotoxin (LAL test)
UniProt accession no.   P15018
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... LIF(3976)

Description

General description

Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line.

Biochem/physiol Actions

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts, and inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B-cell lymphoma-extra large) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.

Physical form

Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Hematopoietic Cytokines

Hematopoietic cytokines are large family of extracellular ligands that stimulate hematopoietic cells to differentiate into eight principle types of blood cells. Numerous cytokines are involved in the...
Keywords: Cell proliferation, Clinical, Growth factors, Ligands

Related Content

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) belongs to the interleukin-6 cytokine family. LIF binds to a heterodimeric receptor consisting of the low-affinity LIF receptor and gp130, with downstream signals bei...
Keywords: Cell culture, Culture media, Growth factors, Transcription

Peer-Reviewed Papers
15

References

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