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SRP6298 Sigma-Aldrich

LAG3/CD223 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym: FDC

  •  NACRES NA.32

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Properties

biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized
mol wt   calculated mol wt 47 kDa
  observed mol wt 60 kDa (DTT-reduced. Protein migrates due to glycosylation. Leu 23 is the predicted N-terminus.)
packaging   pkg of 10 and 50 μg
conjugate   6-His tagged (C-terminus)
UniProt accession no.   P18627
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... LAG3(3902)

Description

General description

LAG3 (lymphocyte activation gene 3), first identified in activated natural killer cells (NK cells), is a transmembrane protein belonging to the Ig (immunoglobulin) superfamily. It is composed of four extracellular Ig-like domains, and the outermost domain has an unusual loop-like insertion. This cell is expressed in activated NK and T cells.

Biochem/physiol Actions

Studies in mice with null mutation for LAG3 (lymphocyte activation gene 3) gene show that this protein is involved in pathways involving natural killer (NK) cells, as in these mice, NK cells are incapable of killing certain tumor targets. This protein is expressed by activated CD4+ T cells, which results in the production of IFN-γ (interferon). This protein is thought to interact with MHC II (major histocompatibility class) molecules on immature DCs (dendritic cells), thus activating and maturing APCs (antigen presenting cells).

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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