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SRP6405 Sigma-Aldrich

ErbB2/HER2/CD340 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym: ERBB2, HER-2/neu, MLN19, NEU, NGL, TKR1

  •  NACRES NA.32

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Properties

Related Categories CD160 to CD340, Cell Biology, Cell Signaling and Neuroscience, Cluster of Differentiation (CD) Proteins, Cytokines and Growth Factors,
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized
potency   5-45 ng/mL
mol wt   calculated mol wt 72.4 kDa
  observed mol wt 90-110 kDa (DTT-reduced. Protein migrates due to glycosylation.)
packaging   pkg of 10 and 50 μg
impurities   <1 EU/μg endotoxin (LAL test)
conjugate   6-His tagged (C-terminus)
UniProt accession no.   P04626
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... ErbB2(2064)

Description

General description

Human epidermal growth factor receptor 2 (HER2), or erb-b2 receptor tyrosine kinase 2 (ErbB2) or tyrosine kinase receptor-1 (tkr-1), is encoded by the gene mapped to human chromosome 17. The encoded protein belongs to the epidermal growth factor family of receptor tyrosine kinases (ErbBs). HER2 membrane protein is characterized with a cysteine-rich extracellular ligand-binding domain, a hydrophobic membrane spanning region and an intracellular tyrosine kinase domain. This protein does not have a ligand, and it either forms heterodimers with other family members or homodimer with itself when expressed at very high levels, to get activated. HER2 is expressed at low level in normal tissues, but at high level in breast cancer tissues.

Biochem/physiol Actions

Human epidermal growth factor receptor 2 (HER2) signaling pathway stimulates cell proliferation and survival in the majority of breast cancers. Thus, overexpression of this protein causes breast cancer. Heterodimer complex of HER2 and phosphatidylinositide 3-kinase (PI3K) is the most potent stimulator of the PI3K/AKT anti-apoptosis pathway. Increased expression of HER2 is also observed in ovarian, colorectal, pancreatic, endometrial and gastric cancers. Trastuzumab, an antibody, works by binding to a domain in the external domain of HER2. This domain is missing in p95, a truncated form of HER2, and hence these cancer cells show resistance to trastuzumab. HER2 protein can be used as a prognostic marker and as a therapeutic option for gynecologic cancers.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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