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SRP6406 Sigma-Aldrich

VEGFR2/Flk-1/KDR human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym: CD309, VEGFR, kinase insert domain receptor

  •  NACRES NA.32

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, Human recombinant VEGF proteins and receptors, Reagents and Supplements, Vascular Endothelial Growth Factor (VEGF) More...
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized
mol wt   calculated mol wt 84.1 kDa
  observed mol wt 100-110 kDa by SDS-PAGE (DTT-reduced. Protein migrates due to glycosylation.)
packaging   pkg of 100 μg
  pkg of 20 μg
impurities   <1 EU/μg endotoxin (LAL test)
conjugate   6-His tagged (C-terminus)
UniProt accession no.   P35968
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... VEGFR2(3791)

Description

General description

Kinase insert domain receptor (KDR) also known as CD309, FLK1, VEGFR, VEGFR2, and is one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). There are three main subtypes of VEGFR, numbered 1, 2 and 3. The VEGF receptors have an extracellular portion consisting of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion containing a split tyrosine-kinase domain. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF. The function of VEGFR-1 is less well defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding (this appears to be particularly important during vasculogenesis in the embryo). In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi′s sarcoma lesions.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Vascular Endothelial Growth Factor (VEGF)

Background VEGF Pathway    Ligands    Receptors VEGF – Signal Transduction Factors Regulating VEGF and VEGFR Expression VEGFR in Human Diseases Conclusion
Keywords: Angiogenesis, Apoptosis, Cancer, Cell proliferation, Diseases, Gene expression, Growth factors, Infrared spectroscopy, Ligands, Transcription, Transduction

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