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SRP6414 Sigma-Aldrich

Cathepsin B human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym: APPS, CPSB, CTSB

  •  NACRES NA.32

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Properties

biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized
mol wt   calculated mol wt 29 kDa (Mature)
  calculated mol wt 36.7 kDa (Pro)
  observed mol wt 34 kDa
  observed mol wt 43_kDaand34 kDa (DTT-reduced. Protein migrates due to glycosylation. Arg18 (pro) or Phe74 (mature) is the predicted N-terminal.)
packaging   pkg of 10 μg
impurities   <1 EU/μg endotoxin (LAL test)
conjugate   6-His tagged (C-terminus)
UniProt accession no.   P07858
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CTSB(1508)

Description

General description

Cathepsin B (CTSB) also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.

Physical form

Lyophilized from 0.22 μm filtered solution in 50 mM Tris-HCl and 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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