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SRP6458 Sigma-Aldrich

TFPI human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym: EPI, LACI, TFI, TFPI1

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Enzyme Inhibitors, Enzyme Inhibitors by Enzyme, Enzymes, Inhibitors, and Substrates, R to Z More...
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized
potency   <0.35 nM IC50
mol wt   calculated mol wt 30 kDa
  observed mol wt 41-45 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 29 is the predicted N-terminal.)
packaging   pkg of 10 μg
impurities   <1 EU/μg endotoxin (LAL test)
conjugate   6-His tagged (C-terminus)
UniProt accession no.   P10646
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... TFPI(7035)

Description

General description

The TFPI (tissue factor pathway inhibitor) gene is mapped to human chromosome 2q32.1. This protein exists in two isoforms TFPIα and TFPIβ.

Tissue factor pathway inhibitor (TFPI) also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.

Biochem/physiol Actions

Inhibition of tissue factor function and thrombus formation by TFPI (tissue factor pathway inhibitor) is a major reason for ischemic stroke in carotid artery disease. The main function of TFPI is to regulate blood coagulation system. It possess both anticoagulant and antimetastatic action. This protein is prominently localized to medial and neointimal smooth muscle cells, macrophages and T-cells of atherosclerotic plaques. Upregulation of the TFPI gene is observed in atherosclerosis. Mutation in the TFPI gene leads to the risk of coronary artery disease.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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