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SRP6465 Sigma-Aldrich

Lipocalin-2/LCN2 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym: 24p3, MSFI, NGAL, Neutrophil Gelatinase-associated Lipocalin, Oncogene 24p3, Siderocalin

  •  NACRES NA.32

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Properties

biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized
mol wt   calculated mol wt 22 kDa
  observed mol wt 23-25 kDa (DTT reduced. Gln 21 is the predicted N-terminal.)
packaging   pkg of 10 and 50 μg
impurities   <1 EU/μg endotoxin (LAL test)
conjugate   6-His tagged (C-terminus)
UniProt accession no.   P80188
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... LCN2(3934)

Description

General description

LCN2 (lipocalin 2), also known as oncogene 24p3, uterocalin, siderocalin, is a 24kDa secreted glycoprotein. It was initially identified in a mouse kidney cell culture infected with simian virus 40 (SV-40). It is a member of the transporter protein family called lipocalins which transport small, hydrophobic ligands.

Lipocalin-2 (LCN2), a secreted glycoprotein, is an adipokine. It is expressed in various cells like respiratory cells, intestinal epithelial cells, endothelial cells, renal tubular cells and cardiomyocytes. The gene LCN2 is located on human chromosome 9q34.

Biochem/physiol Actions

LCN2 (lipocalin 2) functions as a biomarker for various inflammatory disorders as well as cancers. It is also thought to be involved in resistance to drug-mediated apoptosis. It interacts with matrix metalloproteinase-9 and promotes invasion, and it also facilitates tumor growth and is linked with poor prognosis. In lung adenocarcinoma this protein facilitates epithelial to mesenchymal transition (EMT), and hence, promotes metastasis and tumorigenesis, which is repealed by BRM270, an NFκB inhibitor (nuclear factor). Urine levels of LCN2 increase post-nephrotoxic and ischemic insult. In patients undergoing off pump coronary bypass surgery (OPCAB), this protein functions as an early biomarker for acute kidney injury.

Lipocalin-2 (LCN2) has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Polymorphisms in the expression of LCN2 are associated with many cancers such as breast, colorectal, pancreatic, ovarian, gastric, thyroid, ovarian, bladder and kidney cancers.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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