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SRP6539 Sigma-Aldrich

NQO1 human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym: Azoreductase, DT-Diaphorase (DTD), Dioxin-inducible 1, Disphorase-4 (DIA4), Menadione Reductase, NAD(P)H Dehydrogenase (Quinine) 1 (NQO1), NAD(P)H Quinone Oxireductase, Phylloquinone Reductase, Quinone Oxidoreductase 1, Quinone Reductase 1 (QR1)

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Properties

biological source   human
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   liquid
mol wt   ~300 kDa (Human NQO1 aa 1-274 is fused at the C-terminus to a His-tag.)
packaging   pkg of 10 and 50 μg
impurities   <0.1 EU/μg endotoxin (LAL test)
UniProt accession no.   P15559
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... NQO1(1728)

Description

General description

NQO1 (NAD(P)H quinone dehydrogenase 1) is an obligate two-electron reductase. It is a cytoplasmic, homodimeric protein, with one FAD molecule per monomer. This gene is localized to human chromosome 16q23. It shows a wide range of tissue expression.

Biochem/physiol Actions

NQO1 (NAD(P)H quinone dehydrogenase 1) uses NADH or NADPH as a reducing cofactor, and is characterized by its inhibition by dicoumarol. It is thought to confer protection against natural and exogenous quinones. It is involved in the conversion of benzene derived quinones to less toxic hydroquinones, and participates in benzene-associated hematotoxicity. It is involved in the reduction and eventual activation of few chemotherapeutic drugs and of environmental carcinogens, such as heterocyclic amines, nitroaromatic compounds, and possibly cigarette smoke particulate. C609T null variant of this gene is linked with breast cancer susceptibility in north Indian patients. NQO1 is up-regulated in serous ovarian carcinoma, and this expression is linked with poor prognosis; hence, this gene has potential as biomarker for the same.

Physical form

0.2 μm-filtered solution in 50 mM Tris-Cl, pH 8.0, containing 1 mM DTT.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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