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SRP6556 Sigma-Aldrich

Thrombin Active (High Activity) from bovine plasma

≥98% (SDS-PAGE)

Synonym: Activated Factor IIa

  •  NACRES NA.32

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Properties

biological source   bovine plasma
assay   ≥98% (SDS-PAGE)
form   lyophilized
potency   >1500 units/mg
mol wt   37 kDa
packaging   pkg of 10,000 units
  pkg of 100,000 units
  pkg of 1000 units
UniProt accession no.   P00735
shipped in   wet ice
storage temp.   −20°C
Gene Information   bovine ... F2(280685)

Description

General description

Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions.

Biochem/physiol Actions

Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity. Thrombin enzyme (Activated Factor IIa) also participates in inflammation and has mitogenic role on vascular cells. It is also involved in the activation of protein C which is needed for the inactivation of many procoagulant enzymes.

Physical form

Sterile filtered and lyophilized with Mannitol and Sodium Chloride.

Reconstitution

Reconstitute in sterile water (100 U/mL) with 0.9% NaCl. It forms a clear solution.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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