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SRP6586 Sigma-Aldrich

Histone H3 (1-136) human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Synonym: Histone H3/I, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/j, Histone H3/k, Histone H3/l

  •  NACRES NA.32

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones, Histones,
biological source   human
recombinant   expressed in E. coli
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
mol wt   15.5 kDa (1-136 aa)
packaging   pkg of 100 and 250 μg
UniProt accession no.   P68431
  P68431
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... HIST1H3A(8350)

Description

General description

The mammalian chromatin is present in nucleosomes, made of histone octamers (H2A,H2B,H3,H4)2. Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A).

Biochem/physiol Actions

Histone proteins are basic building blocks of chromatin. Changes in the circulating H3 levels are associated with death in sepsis patients. Post-translational modifications of histone tails control chromatin organization and gene transcription. Trimethylated histone H3 lysine 4 (H3K4me3) is present at actively transcribed genes and it helps in promotion of gene expression. Histone H3K36 trimethylation (H3K36me3) is lost in various cancers and is linked with poor patient outcome. Dimethylated H3K9 (H3K9me2) is mainly present in euchromatin and is linked with repressed promoter areas. Histone methyltransferase G9a is responsible for hypoxic stress-mediated H3K9me2 in mammalian cells.

Physical form

Lyophilized powder. Recommended buffer is 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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