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T3405 Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

tablet, 1 mg substrate per tablet

Synonym: 4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

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Properties

Related Categories Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Detection, Detection Substrates,
form   tablet
mp   ≥300 °C
storage temp.   2-8°C
SMILES string   Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI   1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
InChI key   NYNRGZULARUZCC-UHFFFAOYSA-N

Description

Application

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
• anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
• anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
• in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

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