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T5516 Sigma-Aldrich

3,3′,5-Triiodo-L-thyronine sodium salt

γ-irradiated, powder, suitable for, suitable for cell culture

Synonym: Liothyronine, O-(4-Hydroxy-3-iodophenyl)-3,5-diiodo-L-tyrosine sodium salt, T3

  • CAS Number 55-06-1

  • Empirical Formula (Hill Notation) C15H11I3NNaO4

  • Molecular Weight 672.96

  •  Beilstein/REAXYS Number 8179867

  •  EC Number 200-223-5

  •  MDL number MFCD00002594

  •  PubChem Substance ID 24900290

  •  NACRES NA.77

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines, Growth Factors and Hormones, Hormones,
Quality Level   200
biological source   synthetic (organic)
sterility   γ-irradiated
assay   ≥90% (HPLC)
form   powder
application(s)   cell culture | mammalian: suitable
suitability   suitable for
shipped in   ambient
storage temp.   −20°C
SMILES string   [Na+].N[C@@H](Cc1cc(I)c(Oc2ccc(O)c(I)c2)c(I)c1)C([O-])=O
InChI   1S/C15H12I3NO4.Na/c16-9-6-8(1-2-13(9)20)23-14-10(17)3-7(4-11(14)18)5-12(19)15(21)22;/h1-4,6,12,20H,5,19H2,(H,21,22);/q;+1/p-1/t12-;/m0./s1
InChI key   SBXXSUDPJJJJLC-YDALLXLXSA-M
Gene Information   human ... THRA(7067), THRB(7068)
rat ... Thra(81812), Thrb(24831)

Description

General description

3,3′,5′-Triiodo-L-thyronine (T3) is an endocrine hormone produced by the thyroid gland and is regarded as an active thyroid hormone at cellular level. Thyroxine (T4) serves as a precursor for T3 and is generated in extrathyroidal tissues.

Application

3,3′,5-Triiodo-L-thyronine sodium salt has been used:
• as a supplement in colon organ culture media
• to stimulate HuH-7 cells for analysing the hormonal influence on MBL synthesis
• to study its effect on human growth hormone (GH) versus mouse GH expression in the presence of ′′intact′′ GH genes and in a common pituitary cell context

Biochem/physiol Actions

3,3′,5′-Triiodo-L-thyronine (T3) is a thyroid hormone that increases rates of proteins synthesis, stimulates the breakdown of cholesterol, and effects embryonic development. In cell culture, T3 regulates cell differentiation and protein expression. The interaction of T3 with nonhistone proteins in the chromatin initiates the thyroid hormone activity. Thyroid hormones regulate the differentiation and development of tissues and affect a number of metabolic processes. The level of circulating T3 hormone is reduced in hypothyroidism.

Physical form

powder-0 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw, solution stable 2-8 °C, 30 days

Preparation Note

Package size based on 3,3′,5-Triiodo-L-thyronine sodium salt content; vial may also contain inert salts, such as sodium chloride

Reconstitution

To prepare 20 μg/ml stock solution: add 1 ml 1 N NaOH; gently swirl to dissolve; add 49 ml sterile medium.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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1
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Keywords: Adhesion, Atomic absorption spectroscopy, Cell culture, Cell proliferation, Central Nervous System, Clinical, Culture media, Diseases, Gene expression, Growth factors, Hormones, Neurodegenerative Diseases, Pharmaceutical, Phase transitions, RNA immunoprecipitation, Transfection

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Peer-Reviewed Papers
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References

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