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T6146 Sigma

Trypan Blue

powder, BioReagent, suitable for cell culture

Synonym: Direct blue 14

  • CAS Number 72-57-1

  • Linear Formula C34H24N6O14S4Na4

  • Molecular Weight 960.81

  •  Colour Index Number 23850

  •  Beilstein/REAXYS Number 4360496

  •  EC Number 200-786-7

  •  MDL number MFCD00003969

  •  PubChem Substance ID 57654686

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Properties

Related Categories Apoptosis and Cell Cycle, Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Base Ingredients for Media, Cell Analysis, Cell Biology,
InChI Key   GLNADSQYFUSGOU-UHFFFAOYSA-J
product line   BioReagent
form   powder
composition   Dye content, ~40%
mp   >300 °C (lit.)
solubility   H2O: 10 mg/mL
suitability   suitable for cell culture
Gene Information   human ... PTPN1(5770), PTPRF(5792)

Description

General description

The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.

Application

Trypan blue has been used:
• in cell viability assay
• to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
• to stain plant tissue in order to to visualize cell death

Packaging

5, 25, 100 g in glass bottle

Biochem/physiol Actions

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
3
RTECS 
QJ6475000

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Keywords: Cancer, Cell disruption, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Indicators, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Protocols

Cultrex® 3-D Spheroid Fluorometric Proliferation/Viability Assay Protocol

Current in vitro tumor models lack either a physiological context and/or reproducible format for evaluating tumor cells in vitro. At present, the most popular method for compound screening and pathwa...
Keywords: Cancer, Cell culture, Cell proliferation, Diagnostic, Diffusion, Gene expression, Genetic, Microscopy

Cultrex® 96-Well 3D Spheroid BME Cell Invasion Assay Protocol

The 96 Well 3D Spheroid BME Cell Invasion Assay offers a standardized, three dimensional, high content format for quantitating the degree to which invasive cells penetrate a barrier, consisting of ba...
Keywords: Adhesion, Cancer, Cell culture, Centrifugation, Diagnostic, Genetic, Microscopy, Polymerization reactions

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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