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T7254 Sigma-Aldrich

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride

≥96% (TLC), powder

Synonym: (3S)-1-Chloro-3-tosylamido-7-amino-2-heptanone hydrochloride, (3S)-7-Amino-1-chloro-3-tosylamino-2-heptanone hydrochloride, TLCK, Tosyl-L-lysyl-chloromethane hydrochloride

  • CAS Number 4272-74-6

  • Empirical Formula (Hill Notation) C14H21ClN2O3S · HCl

  • Molecular Weight 369.31

  •  Beilstein/REAXYS Number 7106867

  •  EC Number 224-266-4

  •  MDL number MFCD00065395

  •  PubChem Substance ID 24278752

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Description

Application

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride has been used:
• in chymotrypsin purification to prevent binding of trypsins to the affinity support by inhibiting them in the crude extract
• as a protease inhibitor in tissue and cell homogenization
• as a trypsin inhibitor to determine the specific protease activity levels

Biochem/physiol Actions

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli.

Features and Benefits

This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Caution

Stable at least two years when stored desiccated at −20°C.

Legal Information

LOPAC is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
UN 3335
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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