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T9326 Sigma-Aldrich

Thrombin human

BioUltra, recombinant, expressed in HEK 293 cells, aqueous solution, ≥95% (SDS-PAGE)

Synonym: Factor IIa

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
Quality Level   200
recombinant   expressed in HEK 293 cells
product line   BioUltra
assay   ≥95% (SDS-PAGE)
form   aqueous solution
activity   ≥2,000 units/mg protein
mol wt   37.4 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... F2(2147)

Description

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess in vitro hemostatic properties of French lyophilized plasma.

Packaging

150 units in glass bottle

Unit Definition

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

Physical form

supplied as a solution in 20 mM MES, pH 6.0, 500 mM choline chloride

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Peer-Reviewed Papers
15

References

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