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TrueGel3D Hydrogel Kits

CD cell-degradable crosslinker and RGD peptide

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Description

Components

TRUE1-1KT contains lyophilized RGD degradable polymer and CD cell-degradable crosslinker with reconstitution buffer and water.

Application

Suitable for 3D-culture of various cell types, spheroids and tissue samples. It has been used for cell migration and invasion studies, co-culture experiments (tumor cells with fibroblasts) and cell growth and differentiation studies.

Caution

Avoid prolong exposure of CD cell-degradable crosslinker to air.

Features and Benefits

• Easy to use and pre-configured
• Gel with soft stiffness of 400 Pa
• Contains RGD degradable polymer for cell attachment
• Allow cell recovery and post-culture analyses

General description

The gel is formed by crosslinking of modified RGD degradable polymer with CD cell-degradable crosslinker. At physiological pH, the thiol groups of CD cell-degradable crosslinker interact with thiol reactive groups on RGD degradable polymer to form soft gel. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

Gel can be further dissolved by TrueGel3D Enzymatic Cell Recovery Solution (TRUEENZ-500UL) for post-culture analysis including RT-PCR, western blots and other molecular biological studies.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Hydrogels for 3D cell culture

Cells in their natural environment are surrounded by a complex network of extracellular molecules called Extracellular Matrix (ECM). This network provides structure and function, in the form of bioch...
Keywords: Adhesion, Cancer, Cell culture, Growth factors

True Gel3D™ Troubleshooting guide

From our library of Articles, Sigma-Aldrich presents True Gel3D™ Troubleshooting guide
Keywords: Adhesion

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a biochemically defined hydrogel formed by mixing polymers with crosslinkers. Compared to other hydrogels based on a biological extract from animal cells (e.g. mouse EHS tumor cells), T...
Keywords: Adhesion, Buffers, Cancer, Cell culture, Growth factors, Immobilization, Immunostaining, Ligands, Microscopy, PAGE, SDS PAGE

Protocols

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

The identity and concentration of peptides or proteins can be varied when constructing a TrueGel 3D™ hydrogel. This peptide concentration optimization may be important for specific applications such ...
Keywords: Adhesion, High performance liquid chromatography, Ligands, Oxidations

TrueGel3D™ Fast Gelling Hydrogels Preparation Protocol

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimi...
Keywords: Adhesion, Buffers, Cell attachment, Cell culture, Crystallization, Oxidations

TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) preparation protocol

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel enables the encapsulation of cells to permit their growth in a 3-dimensional environment to bett...
Keywords: Adhesion, Cell culture, Oxidations

Related Content

Beating Cardiac Muscle Cells Cultured in TrueGel3D™ Synthetic Hydrogels

A suspension of freshly prepared cells from embryonic chick heart tissue was embedded in a Dextran-based hydrogel modified with RGD Peptide and crosslinked with matrix metalloprotease-cleavable cross...

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