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TrueGel3D Hydrogel Kits

FAST-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker

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Description

Components

TRUE3-1KT contains lyophilized FAST-DEXTRAN (polymer) and CD cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.

Application

TRUE3-1KT is used for cell migration studies of amoeboid cells including neutrophils, dendritic cells and lymphocytes. It has been used to study the effect of mechanic stimulus over tumor development, tumor progression and cell differentiation.

Caution

Avoid prolonged exposure of CD cell-degradable crosslinker to air.

Features and Benefits

• Fast gelling (takes few seconds to minutes after addition of crosslinker)
• Contains MMP-cleavable crosslinker
• Allow cell recovery and post-culture analyses
• Flexibility in modulating gel stiffness
• Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)

General description

The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on CD cell-degradable crosslinker. Malemide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Safety & Documentation

Safety Information

RIDADR 
UN 1789 8 / PGIII
Protocols & Articles

Articles

Co-culture of Tumor and Stromal Cells in TrueGel3D™ Hydrogels

Tumor microenvironment (TME) is a complex aggregation of malignant cells and non-transformed cells (fibroblasts, neuroendocrine cells, blood and inflammatory cells). The cells within TME have dynamic...
Keywords: Adhesion, Apoptosis, Cancer, Cell culture, Microscopy, Optical microscopy, Polymerase chain reaction

Hydrogels for 3D cell culture

Cells in their natural environment are surrounded by a complex network of extracellular molecules called Extracellular Matrix (ECM). This network provides structure and function, in the form of bioch...
Keywords: Adhesion, Cancer, Cell culture, Growth factors

True Gel3D™ Troubleshooting guide

From our library of Articles, Sigma-Aldrich presents True Gel3D™ Troubleshooting guide
Keywords: Adhesion

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a biochemically defined hydrogel formed by mixing polymers with crosslinkers. Compared to other hydrogels based on a biological extract from animal cells (e.g. mouse EHS tumor cells), T...
Keywords: Adhesion, Buffers, Cancer, Cell culture, Growth factors, Immobilization, Immunostaining, Ligands, Microscopy, PAGE, SDS PAGE

Protocols

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

The identity and concentration of peptides or proteins can be varied when constructing a TrueGel 3D™ hydrogel. This peptide concentration optimization may be important for specific applications such ...
Keywords: Adhesion, High performance liquid chromatography, Ligands, Oxidations

TrueGel3D™ Fast Gelling Hydrogels Preparation Protocol

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimi...
Keywords: Adhesion, Buffers, Cell attachment, Cell culture, Crystallization, Oxidations

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