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XNAPG Sigma-Aldrich

SYBR® Green Extract-N-Amp Plant PCR Kit

sufficient for 100 preparations

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Kits, Fluorescent Probes, Labels, Particles and Stains More...
usage   sufficient for 100 preparations
shipped in   wet ice
storage temp.   −20°C

Description

General description

The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated. The kits contain all necessary reagents for both extraction and amplification.

Two solutions combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.

Other Notes

The protocol uses a 20 μl PCR reaction.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. SYBR Green nucleic acid stain is licensed from Molecular Probes, Inc. under US Patent No. 5,436,134 for use in Real-Time PCR by end-users for research and development only. SYBR is a registered trademark of Molecular Probes, Inc.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays. Eliminate the need for any columns or long enzymatic sample puri...
Keywords: Amplification, Buffers, Digestions, Homogenization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Solving the Space Constraints of High-Throughput Genotyping

High-throughput genotyping is a critical tool for modern plant breeding programs. The more samples analyzed, the more marker data collected, and the better the chances to identify traits that enhance...
Keywords: Agriculture, Sample preparations

Peer-Reviewed Papers
15

References

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Add to Cart

XNAP REDExtract-N-Amp Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
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XNAP2 Extract-N-Amp Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
XNAR Extract-N-Amp Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications
XNAPR REDExtract-N-Amp Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications
XNAPS REDExtract-N-Amp Plant PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications
R4775 REDExtract-N-Amp PCR ReadyMix, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
E3004 Extract-N-Amp PCR ReadyMix, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
E8155 Extract-N-Amp PCR Diluent
D5688 Dilution Solution, Extract-N-Amp Plant Dilution Solution
S4320 SYBR® Green Extract-N-Amp PCR ReadyMix, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
E0781 Extract-N-Amp PCR ReadyMix with qPCR Reference dye

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