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XNAS2 Sigma-Aldrich

Extract-N-Amp Seed PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

Purchase

Properties

Related Categories DNA & RNA Purification, Extract-N-Amp Plant PCR Kits, Extract-N-Amp Seed PCR Kits, Genomic DNA Purification, Molecular Biology,
usage   sufficient for 100 amplifications
  sufficient for 100 extractions
Featured Industry   Agriculture
shipped in   dry ice
storage temp.   −20°C

Description

Features and Benefits

• Perfect for quick genotyping results
• Rapid extraction of genomic DNA for PCR in 15 minutes
• No long enzymatic digestions
• Hot Start antibody for highly specific PCR amplification of genomic DNA

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Application

Extract-N-Amp seed PCR Kit has been used for DNA extraction.

General description

The Extract-N-Amp Seed PCR Kit provides all reagents necessary to rapidly extract DNA from a wide variety of plant seeds and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays. Eliminate the need for any columns or long enzymatic sample puri...
Keywords: Amplification, Buffers, Digestions, Homogenization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing

Extract-N-Amp™ Seed PCR Kit

Description Procedure Application Beneficial Features Ordering Information Kit Contents Sample Data Materials
Keywords: Amplification, Centrifugation, DNA purification, Filtration, Polymerase chain reaction, Precipitation, Purification

Improving Real-Time PCR Success

To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Solving the Space Constraints of High-Throughput Genotyping

High-throughput genotyping is a critical tool for modern plant breeding programs. The more samples analyzed, the more marker data collected, and the better the chances to identify traits that enhance...
Keywords: Agriculture, Sample preparations

Protocols

Extract-N-Amp Seed PCR Protocol

Product Description Reagents provided Reagents and equipment required, not provided Precautions and Disclaimer Storage Procedures Related Products Troubleshooting Guide Materials References
Keywords: Amplification, Diagnostic, Evaporation, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Precipitation, Purification, Sequencing

Related Content

Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

Plant Breeding Workflow - Collaborating to Empower Yield Improvements

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...
Keywords: Building blocks, Mass spectrometry, Organic synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Vitamins

Peer-Reviewed Papers
15

References

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