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Y2146 Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, and tryptophan

Synonym: Yeast Synthetic Drop-out Media Supplements

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Description

Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Introduction to Yeast Media

Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced. They are relatively easy to grow under laboratory conditions. Moreover, despite thei...
Keywords: Culture media, Fermentation, Gene expression, Indicators, Molecular biology, Sterilizations, Transcription, Vitamins, transformation

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. We are an essential part of your...
Keywords: Cell disruption, Gene expression, transformation

Yeast Drop Out Bulletin

Catalog Number Y1501: without uracil Catalog Number Y1751: without histidine Catalog Number Y1896: without lysine Catalog Number Y1376: without leucine Catalog Number Y1876: without tryptophan Catalo...
Keywords: Filtration, transformation

Protocols

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Culture media, Detergents, Genetic, transformation

Peer-Reviewed Papers
15

References

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Description

Add to Cart

Y2001 Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan and uracil
Y1376 Yeast Synthetic Drop-out Medium Supplements, without leucine
Y0626 Yeast Nitrogen Base Without Amino Acids, Yeast classification medium
G7021 D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Y1771 Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan
Y1896 Yeast Synthetic Drop-out Medium Supplements, without lysine
Y2021 Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine
A5306 Bacteriological agar, for molecular biology

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