• USA Home
  • ZMS1019 - Anti-Olig2 Antibody, clone 211F1.1 ZooMAb® Mouse Monoclonal

EMAIL THIS PAGE TO A FRIEND
ZMS1019 Sigma-Aldrich

Anti-Olig2 Antibody, clone 211F1.1 ZooMAb® Mouse Monoclonal

Green Alternative Antibody Enhanced Validation

recombinant, expressed in HEK 293 cells

Synonym: Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19, Oligo2, Oligodendrocyte transcription factor 2, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, bHLHb1, bHLHe19

Purchase

Properties

Related Categories 12 Principles Aligned Products, Alphabetical Index, Antibodies, Antibodies (Greener), Chemical Synthesis,
product line   ZooMAb® learn more
conjugate   unconjugated
clone   211F1.1, monoclonal
  recombinant monoclonal
biological source   mouse (recombinant)
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  western blot: suitable
species reactivity   mouse, rat, human
mol wt   calculated mol wt 32.38 kDa
  observed mol wt ~37 kDa
enhanced validation   recombinant expression
Learn more about Antibody Enhanced Validation
form   lyophilized
shipped in   ambient
storage temp.   2-8°C
packaging   antibody small pack of 25 μL
antibody form   purified antibody
isotype   IgG2a
Quality Level   200
recombinant   expressed in HEK 293 cells
antibody product type   primary antibodies
greener alternative product characteristics   Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
greener alternative category   Aligned

Description

General description



ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 211F1.1 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Oligodendrocyte transcription factor 2 (Olig2).

Immunogen

Recombinant human Oligodendrocyte transcription factor 2 (Olig2).

Application

Anti-Olig2, clone 211F1.1 ZooMAb, Cat. No. ZMS1019, is a recombinant Mouse monoclonal antibody that targets Oligodendrocyte transcription factor 2 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Olig2 in rat brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Olig2 in human cerebral cortex tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Olig2 in Oligodendrocyte precursor cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Oligodendrocyte transcription factor 2 (UniProt: Q13516; also known as Oligo2, Class B basic helix-loop-helix protein 1, bHLHb1, Class E basic helix-loop-helix protein 19, bHLHe19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, Olig2) is encoded by the OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) gene (Gene ID: 10215) in human. Olig2 is a basic helix-loop-helix (bHLH) transcription factor that is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It is expressed in oligodendrocytes and stronger expression is reported in oligodendrogliomas. It functions together with ZNF488 to promote oligodendrocyte differentiation and cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. A chromosomal aberration involving OLIG2 is reported to cause a form of T-cell acute lymphoblastic leukemia (T-ALL). OLIG2 expression is induced by sonic hedgehog and fibroblast growth factor during development. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?