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  • ZRB457 - Anti-c-Fos Antibody, clone 1D10, ZooMAb® Rabbit Monoclonal

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ZRB457 Sigma-Aldrich

Anti-c-Fos Antibody, clone 1D10, ZooMAb® Rabbit Monoclonal

Green Alternative Antibody Enhanced Validation

recombinant, expressed in HEK 293 cells

Synonym: Cellular oncogene fos, G0/G1 switch regulatory protein 7, Proto-oncogene c-Fos

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Properties

Related Categories 12 Principles Aligned Products, Alphabetical Index, Antibodies, Antibodies (Greener), CE-CH,
product line   ZooMAb® learn more
conjugate   unconjugated
clone   1D10, monoclonal
  recombinant monoclonal
biological source   rabbit (recombinant)
application(s)   immunocytochemistry: 1:100-1:250
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100
  western blot: suitable using 1:1,000
species reactivity   rat, human
mol wt   calculated mol wt 40.7 kDa
  observed mol wt ~56 kDa
enhanced validation   recombinant expression
Learn more about Antibody Enhanced Validation
form   lyophilized
shipped in   ambient
storage temp.   2-8°C
packaging   antibody small pack of 25 μL
antibody form   purified antibody
isotype   IgG
recombinant   expressed in HEK 293 cells
antibody product type   primary antibodies
greener alternative product characteristics   Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
UniProt accession no.   P01100
Gene Information   human ... FOS;G0S7(2353)

Description

General description




ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1D10 a ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects c-Fos. It targets an epitope with in 14 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human c-Fos.

Application

Anti-c-Fos, clone 1D10 ZooMAb, Cat. No. ZRB457, is a recombinant Rabbit Monoclonal Antibody that specifically targets Proto-oncogene c-Fos and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected c-Fos in serum starved HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected c-Fos in rat brain tissue sections.

Target description

Proto-oncogene c-Fos (UniProt: P01100; also known as Cellular oncogene fos, G0/G1 switch regulatory protein 7) is encoded by the FOS (also known as G0S7) gene (Gene ID: 2353) in human. C-Fos is a nuclear phosphoprotein that forms a tight, but non-covalently linked, complex with the JUN/AP-1 transcription factor. In the heterodimer, c-Fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. In quiescent cells, the small amount of c-Fos present is phosphorylated at tyrosine 10 and 30. This tyrosine phosphorylated form is cytosolic. Following induction of cell growth, it first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum is shown to require dephosphorylation at tyrosine 10 and 30. In growing cells, c-Fos is dephosphorylated by PTPN2, which leads to its association with endoplasmic reticulum membranes and activation of phospholipid synthesis. A basic motif (aa 139-159) is required for the activation of phospholipid synthesis. Upon stimulation by nerve growth factor it is undergoes phosphorylation in the C-terminal region by MAPK and RSK1. Phosphorylation on both serine 362 and serine 374 leads to its stabilization. Phosphorylation on serine 362 and serine 374 primes further phosphorylation on threonine 325 and 331 through promoting docking of MAPK to the DEF domain. c-Fos is also reported to be constitutively sumoylated with SUMO1, SUMO2 and SUMO3 and sumoylation requires heterodimerization with JUN and is enhanced by mitogenic stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on threonine 232. Three different isoforms of c-Fos have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

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