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  • ZRB5535 - Anti-SOX-9 Antibody, clone 2B10, ZooMAb® Rabbit Monoclonal

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ZRB5535 Sigma-Aldrich

Anti-SOX-9 Antibody, clone 2B10, ZooMAb® Rabbit Monoclonal

Green Alternative Antibody Enhanced Validation

recombinant, expressed in HEK 293 cells

Synonym: Transcription factor SOX-9

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Properties

Related Categories 12 Principles Aligned Products, Alphabetical Index, Antibodies, Antibodies (Greener), Chemical Synthesis,
product line   ZooMAb® learn more
conjugate   unconjugated
clone   2B10, monoclonal
  recombinant monoclonal
biological source   rabbit (recombinant)
application(s)   immunocytochemistry: suitable using 1:100
  immunohistochemistry: suitable using 1:100
  western blot: suitable using 1:1,000
species reactivity   mouse, human
species reactivity (predicted by homology)   rat
mol wt   calculated mol wt 56.14 kDa
  observed mol wt ~65 kDa
enhanced validation   recombinant expression
Learn more about Antibody Enhanced Validation
form   lyophilized
shipped in   ambient
storage temp.   2-8°C
packaging   antibody small pack of 25 μL
antibody form   purified antibody
isotype   IgG
recombinant   expressed in HEK 293 cells
antibody product type   primary antibodies
greener alternative product characteristics   Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
UniProt accession no.   P48436
Gene Information   human ... SOX9(6662)

Description

General description




ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 2B10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human and murine SOX-9. It targets an epitope with in 24 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human SOX-9.

Application

Anti-SOX-9, clone 2B10, ZooMAb is a recombinant Rabbit Monoclonal antibody that detects Sox-9 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SOX-9 in HepG2 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SOX-9 in SW480 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SOX-9 in E16.5 mouse embryo, mouse nasal cavity, and developing mouse gut tissue sections.

Target description

Transcription factor SOX-9 (UniProt P48436; also known SOX-9) is encoded by the SOX9 gene (Gene ID: 6662) in human. SOX family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences encoding for the HMG-box domains that mediate sequence-specific DNA binding. SOX genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of SOX genes cluster at about 40 genomic loci and at least 30 SOX genes have been identified. SOX-9 contains one HMG box DNA-binding domain and acts as a key regulator of developmental processes, including male sex determination, normal skeletal development, chondrogenesis, neurogenesis, and neural crest development. SOX-9 is acts downstream of sonic hedgehog signaling and is shown to be essential for the maintenance of multipotent neural stem cells in embryonic and adult central nervous system (CNS). It is not detected in neuroblasts of the adult subependymal zone (SEZ); however, it is expressed by transit amplifying cells and ependymal cells in the same region. SOX-9 is also shown to be essential for the ′switch′ from neurogenesis to gliogenesis in caudal regions of the developing CNS. Up-regulation of SOX-9 is reported in cancers of various origins, including breast, colon, prostate, and lung, where its expression correlates with malignancy and patient survival. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Scott, CE et al. (2010). Nat. Neurosci. 13(10); 1181-89).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

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