• USA Home
  • A0224 - Anti-l/s-Afadin antibody produced in rabbit

A0224 Sigma-Aldrich

Anti-l/s-Afadin antibody produced in rabbit

affinity isolated antibody



Related Categories AE-AK, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.
  indirect immunofluorescence: 1:250 using cultured dog (MDCK) cells and of cultured human HepG2 cells
  indirect immunofluorescence: 1:500 using frozen sections of mouse liver
  microarray: suitable
  western blot: 1:1000 using extract of rat brain.
species reactivity   canine, rat, mouse, human
mol wt   antigen ~200 kDa (and 190 kDa)
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
Gene Information   human ... MLLT4(4301)
mouse ... Mllt4(17356)
rat ... Mllt4(26955)


General description

Afadin is a cell-cell adherens junction, F-actin binding multidomain protein. This adherens junction protein has two splicing variants: the ubiquitously expressed l-afadin, and the smaller s-afadin, which is abundantly expressed in neural tissues. Afadin associates with E-catenin proteins.

S-Afadin is a protein which lacks both the third proline-rich and the F-actin binding regions and is homologous to the human AF-6 (ALL-1 fusion partner from chromosome 6) gene product. Anti-l/s-Afadin is developed in rabbit using a synthetic peptide of rat afadin.


Anti-l/s-Afadin specifically binds to l-afadin and s-afadin by immunoblotting (approximately 200 and 190 kDa respectively). Additional bands may appear in certain extract preparations in western blot assays. The immunizing peptide specifically inhibits the staining of the I/s-afadin bands. The antibody is specific for human, dog, rat, and mouse l/s-afadin.


synthetic peptide corresponding to rat l/s afadin sequence, amino acids 1163-1179, with N-terminal cysteine added, conjugated to BSA.


Anti-l/s-Afadin antibody produced in rabbit has been used in :
•  immunocytochemistry
• immunohistochemistry
• immunoprecipitation

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

s-Afadin binds to several cytoplasmic proteins such as the small guanosine triphosphatase (GTPase) Ras, the tight junction protein ZO-1 2 and the vinculin binding protein ponsin/ sorbin and SH3 domain containing 1 (SH3P12). In addition, l-afadin also binds through its PDZ several integral membrane components such as the Ca2+ -independent homophilic immunoglobulin-like nectin, the junctional adhesion molecule (JAM)4 and a subset of the EphB receptor protein-tyrosine kinases.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?