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A0410 Sigma-Aldrich

Aminoallyl-dUTP sodium salt

≥85%, lyophilized powder

Synonym: 5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP

  • CAS Number 936327-10-5

  • Empirical Formula (Hill Notation) C12H20N3O14P3 · xNa+

  • Molecular Weight 523.22 (free acid basis)

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Description

Application

Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.

Aminoallyl-dUTP sodium salt has been used:
• to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
• for the generation of chromatin immunoprecipitation (ChIP) probes
• in the reverse transcription of RNA for the synthesis of fluorescent labelled probes

Biochem/physiol Actions

Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles
Peer-Reviewed Papers
15

References

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