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A0480 Sigma-Aldrich

Monoclonal Anti-Actin (plant) antibody produced in mouse

clone 10-B3 (MAbGPa), purified immunoglobulin

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Plant Biology, Primary Antibodies More...
species reactivity   plant
application(s)   immunohistochemistry: suitable
  indirect ELISA: suitable
  western blot: 1-2 μg/mL using corn leaves extracts
clone   10-B3 (MAbGPa), monoclonal
antibody form   purified immunoglobulin
isotype   IgG2b
mol wt   antigen mol wt ~45 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   Arabidopsis thaliana ... ACT8(841347)
biological source   mouse
antibody product type   primary antibodies
conjugate   unconjugated

Description

Immunogen

ACT8 protein.

General description

Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.
Monoclonal Anti-Actin (plant) antibody recognizes actin in several plant species including Dictyostelium. In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11 and 12).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Monoclonal Anti-Actin (plant) antibody is suitable for use in western blot (1-2 μg/mL using corn leaves extracts), indirect ELISA and immunohistochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibody Basics

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Improving Reproducibility: Best Practices for Antibodies

In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.
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Right-sized Antibodies - New Small Pack size Antibodies Available

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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