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A0480 Sigma-Aldrich

Monoclonal Anti-Actin (plant) antibody produced in mouse

clone 10-B3 (MAbGPa), purified immunoglobulin

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Plant Biology, Primary Antibodies More...
conjugate   unconjugated
clone   10-B3 (MAbGPa), monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  indirect ELISA: suitable
  western blot: 1-2 μg/mL using corn leaves extracts
species reactivity   plant
mol wt   antigen ~45 kDa
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   purified immunoglobulin
isotype   IgG2b
Quality Level   200
antibody product type   primary antibodies
Gene Information   Arabidopsis thaliana ... ACT8(841347)

Description

General description

Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.
Monoclonal Anti-Actin (plant) antibody recognizes actin in several plant species including Dictyostelium. In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11 and 12).

Monoclonal Anti-Actin (plant) (mouse IgG2b isotype) is derived from the hybridoma 10-B3 (MabGPa) produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with ACT8 protein. In Arabidopsis, actin is divided into two major phylogenetic classes namely reproductive and vegetative class.

Immunogen

ACT8 protein.

Application

Monoclonal Anti-Actin (plant) antibody produced in mouse has been used in:
• two-layer phos-tag polyacrylamide gel electrophoresis (PAGE)
• immunohistochemistry and enzyme-linked immunosorbent assay (ELISA).
• immunoblotting

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

In Arabidopsis ACT7 isovariant is essential for normal phytohormone response to hormones in different organs. Mutation in the actin (ACT)1 gene is associated with the development of dwarfism in plants in addition to delayed flowering, highly reduced organ size and varied branching pattern of the leaf trichomes and inflorescence stem. In plants, there is a developmental switch in the modulation of actin isovariants throughout cell differentiation and maturation phase.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Improving Reproducibility: Best Practices for Antibodies

In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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