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A0521 Sigma-Aldrich

α-Amylase from human saliva

Green Alternative

Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein

Synonym: 1,4-α-D-Glucan-glucanohydrolase



General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

α-amylase is the main secretory protein constituent of saliva. It contains 496 amino acids and has two isoforms, glycosylated and non-glycosylated. α-amylase gene is located on human chromosome 1p21.1. α-amylase is mainly produced in pancreas and saliva.


α-amylase from human saliva has been used to test α-amylase inhibitors using HPLC (high performance liquid chromatography). It also has been used to study the digestibility of human milk oligosaccharides (HMO).


100, 500, 2500 units in glass bottle

Biochem/physiol Actions

α-amylase catalyses the hydrolysis of α-1,4 glycosidic linkage in oligosaccharides. It plays a crucial role in the initial digestion of starch, glycogen and polysaccharides. Elevated levels of amylase in serum is linked to acute pancreatitis.

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Physical form

Lyophilized powder containing (NH4)2SO4 and sodium citrate

Preparation Note

Chromatographically purified

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Enzymatic Assay of Alpha-Amylase

The spectrophotometric stop reaction determination (A540, Light path = 1 cm) is based on the following reaction:

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