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A0844 Sigma-Aldrich

Anti-AP-2α antibody produced in rabbit

affinity isolated antibody, PBS solution

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Properties

Related Categories AA - CA, AO-AQ, Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 1:100 using cultured human HeLa cells
  microarray: suitable
  western blot: 1:250 using a nuclear extract from HeLa human carcinoma cells
species reactivity   chicken, human, mouse
mol wt   antigen ~50 kDa
form   PBS solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P05549
Gene Information   human ... TFAP2A(7020)

Description

General description

Activation Protein transcription factor 2 (AP-2) is a developmentally modulated transcription factor that binds to specific sequence such as CCCCAGGC or related sequences. These sequences are usually present in several promoter and enhancer gene regions, including the promoters of the AP-2 genes themselves,,. AP-2 expression has also been linked to human breast cancer cells.
By immunoblotting, rabbit anti-AP-2α reacts specifically with human AP-2α. The sequence recognized by the antibody differs from the corresponding mouse sequence by one residue and from the chicken sequence by 2 residues.

Immunogen

synthetic peptide corresponding to human AP-2α, C-terminal sequence, (amino acids 420-437) conjugated to KLH with glutaraldehyde.

Application

Rabbit anti-AP-2alpha antibody can be used for western blot (1:250), microarray and indirect immunofluorescence (1:100) assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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A7107 Monoclonal Anti-AP2 antibody produced in mouse, ~1.0 mg/mL, clone A6/2/2, purified immunoglobulin, buffered aqueous solution

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