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A0855 Sigma-Aldrich

Monoclonal Anti-Auxin antibody produced in mouse

clone 1E11-C11, purified from hybridoma cell culture

Synonym: Anti-IAA, Anti-Indole-3-acetic acid

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Properties

Related Categories ATH-AZ, Alphabetical Index, Antibodies, Plant Biology, Primary Antibodies More...
biological source   mouse
recombinant   expressed in mouse cell line
antibody form   purified immunoglobulin
antibody product type   primary antibodies
clone   1E11-C11, monoclonal
form   PBS solution
species reactivity   plant
application(s)   dot blot: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  indirect ELISA: 1-2 μg/mL using using IAA conjugated to BSA
  western blot: suitable
isotype   IgG2b
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

indoleacetic acid (IAA) carboxyl linked to ovalbumin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Indole-3-acetic acid was identified in developing P.abies spruce plant parrafin embedded formalin-fixed embryos by immunohistochemistry with the monoclonal anti-indole-3-acetic acid antibody.

Mouse monoclonal anti-auxin antibody has been used for immunofluorescence studies. The antibody can also be used for ELISA, dot blot and western blot analyses.

General description

Auxin is a plant hormone that regulates physiological growth and development. This hormone regulates cellular functions such as elongation, turgor, division, and differentiation. Indoleacetic acid (IAA) is recognized as the main auxin in several plants. IAA is synthesized from tryptophan (Trp) using Trp dependent pathways,. Mouse monoclonal anti-auxin antibody recognizes indoleacetic acid but not free unmethylated IAA.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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