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  • A0855 - Monoclonal Anti-Auxin antibody produced in mouse

A0855 Sigma-Aldrich

Monoclonal Anti-Auxin antibody produced in mouse

clone 1E11-C11, purified from hybridoma cell culture

Synonym: Anti-IAA, Anti-Indole-3-acetic acid



Related Categories ATH-AZ, Alphabetical Index, Antibodies, Plant Biology, Primary Antibodies More...
conjugate   unconjugated
clone   1E11-C11, monoclonal
biological source   mouse
application(s)   dot blot: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  indirect ELISA: 1-2 μg/mL using using IAA conjugated to BSA
  western blot: suitable
species reactivity   plant
form   PBS solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2b
Quality Level   200
recombinant   expressed in mouse cell line
antibody product type   primary antibodies


General description

Auxin is a plant hormone that regulates physiological growth and development. This hormone regulates cellular functions such as elongation, turgor, division, and differentiation. Indoleacetic acid (IAA) is recognized as the main auxin in several plants. IAA is synthesized from tryptophan (Trp) using Trp dependent pathways,. Mouse monoclonal anti-auxin antibody recognizes indoleacetic acid but not free unmethylated IAA.


indoleacetic acid (IAA) carboxyl linked to ovalbumin.


Indole-3-acetic acid was identified in developing P.abies spruce plant parrafin embedded formalin-fixed embryos by immunohistochemistry with the monoclonal anti-indole-3-acetic acid antibody.

Mouse monoclonal anti-auxin antibody has been used for immunofluorescence studies. The antibody can also be used for ELISA, dot blot and western blot analyses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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