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A0856 Sigma-Aldrich

Anti-ATG5 (N-terminal) antibody produced in rabbit

affinity isolated antibody, PBS solution

Synonym: ATG5 Antibody - Anti-ATG5 (N-terminal) antibody produced in rabbit, Anti-Apoptosis-specific protein, Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog (S. cerevisiae), Anti-Autophagy protein 5-like, Atg5 Antibody

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Properties

Related Categories AS-ATG, Alphabetical Index, Antibodies, Antibodies for Autophagy, Antibodies for Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunofluorescence: suitable
  western blot: 0.5-1 μg/mL using whole extracts of human K562, rat NRK, and mouse 3T3 cells
species reactivity   human, rat, mouse
mol wt   antigen ~56 kDa (Atg5-Atg12 complex)
form   PBS solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   Q9H1Y0
Gene Information   human ... ATG5(9474)
mouse ... Atg5(11793)
rat ... Atg5(365601)

Description

Specificity

Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.

Immunogen

synthetic peptide corresponding to amino acids 2-15 of human ATG5, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Application

Anti-ATG5 (N-terminal) antibody has been used:
• in immunoblotting
• in immunofluorescence
• in western blotting

Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Small Pack Size Primary Antibodies

Economical means affordable options when exploring new antibodies. To provide you with convenient and economical access to our highly characterized antibodies, we now offer small pack size antibodies...
Keywords: Apoptosis, Cancer, Enzyme-linked immunosorbent assay, Epigenetics, Flow cytometry, Growth factors, Immunocytochemistry, Immunohistochemistry, Immunology, Individual protein Immunoprecipitation, Inflammation, Neuroscience, Research areas, Western blot

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

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A0731 Anti-ATG5 (C-terminal) antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody

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