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A1226 Sigma-Aldrich

Anti-Actopaxin antibody produced in rabbit

IgG fraction of antiserum, PBS solution

Synonym: Anti-α-Parvin, Anti-CH-ILKBP

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   microarray: suitable
  western blot: 1:3,000 using whole cell extract of the human endothelial ECV304 cell line.
species reactivity   mouse, human
mol wt   antigen 42 kDa
form   PBS solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   Q9NVD7
Gene Information   human ... PARVA(55742)
mouse ... Parva(57342)

Description

General description

Actopaxin is a member of actin binding proteins that include beta-parvin/affixin and gamma-parvin. Actopaxin is known to associate with F-actin and paxillin LD motifs LD1 and LD4. This protein also directly binds to serine/threonine integrin-linked kinase (ILK). Studies have reported that actopaxin may be involved in integrin-dependent remodeling of the actin cytoskeleton during cell adhesion, motility and division.
Rabbit anti-actopaxin recognizes actopaxin (42 kDa). Staining of actopaxin in immunoblotting is specifically inhibited with actopaxin immunizing peptide (mouse, amino acids 35-53).

Immunogen

synthetic peptide corresponding to the N-terminal region of mouse actopaxin (amino acids 35-53). The sequence is identical in human actopaxin and has considerable homology (~70%) with β-parvin/affixin, but no homology with γ-parvin.

Application

Actopaxin was detected in HEK 293 cell lysates at 42 kDa by western blot using rabbit anti-actopaxin antibody at a 1:1000 dilution. In these cells, actopaxin associated with an ILK complex.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Rabbit anti-actopaxin can also be used for protein microaarays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
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Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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