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  • A1231 - Anti-Aurora-A Kinase antibody, Mouse monoclonal

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A1231 Sigma-Aldrich

Anti-Aurora-A Kinase antibody, Mouse monoclonal

clone 35C1, purified from hybridoma cell culture

Synonym: Anti-Serine/threonine protein kinase 15, Anti-ARK-1, Anti-AURORA2, Anti-Aurora-related kinase 1, Anti-BTAK, Anti-STK15, Anti-STK6, Monoclonal Anti-Aurora-A Kinase antibody produced in mouse, Monoclonal Anti-Aurora-A Kinase

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Properties

Related Categories ATH-AZ, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   35C1, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  western blot: 0.25-0.5 μg/mL using HeLa total cell extract
species reactivity   mouse, human
mol wt   antigen mol wt ~46 kDa
form   PBS solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2b
antibody product type   primary antibodies
recombinant   expressed in mouse cell line
concentration   ~2 mg/mL
UniProt accession no.   O14965
Gene Information   human ... AURKA(6790), AURKA(6790)
mouse ... Aurka(20878), Aurka(20878)

Description

General description

Aurora-A (Aur-A) kinase is a serine threonine kinase that regulates cell division. Elevated levels of Aur-A have been associated with inhibition of apoptosis, and evasion of spindle assembly checkpoints. Aur-A may also be used as a prognostic marker for nasopharyngeal cancers in humans . Monoclonal Anti-Aurora-A Kinase antibody is specific for Aurora-A Kinase in humans and mice.

Immunogen

recombinant human Aurora-A kinase.

Application

Aurora A kinase is required for proper mitotic spindle organization and localizes to centrosomes at sites of microtubule nucleation in Hela cells. Aurora A kinase is immunoprecipitated from cytoskeletal extracts using monoclonal anti-Aurora A kinase antibody. Aurora B and C are not found expressed on centromeres.

Monoclonal Anti-Aurora-A Kinase antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot, indirect ELISA and western blot (0.25-0.5 μg/mL using HeLa total cell extract). The antibody product does not inhibit kinase activity of Aurora-A kinase, hence the product can be used to measure the in vivo aurora-A kinase activity after immunoprecipitation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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