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A1410 Sigma-Aldrich

Actinomycin D

from Streptomyces sp., ~98% (HPLC)

Synonym: Actinomycin C1, Actinomycin IV, Dactinomycin

  • CAS Number 50-76-0

  • Empirical Formula (Hill Notation) C62H86N12O16

  • Molecular Weight 1255.42

  •  Beilstein/REAXYS Number 605235

  •  EC Number 200-063-6

  •  MDL number MFCD00005033

  •  PubChem Substance ID 24890541

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Properties

Related Categories A - K, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics by Application,
Quality Level   PREMIUM
biological source   Streptomyces sp.
InChI Key   RJURFGZVJUQBHK-IIXSONLDSA-N
assay   ~98% (HPLC)
form   powder
optical activity   [α]/D alpha: 28/D (Rotation: -315 degrees (c=0.25% in methanol))
color   red to orange
  red, powder
mp   251-253
solubility   DMSO: 1 mg/mL
  ethanol: soluble
Mode of action   DNA synthesis | interferes
antibiotic activity spectrum   neoplastics
storage temp.   2-8°C

Description

General description

Actinomycin D is a polypeptide antibiotic first described by Waksman. It is bacteriostatic in nature, especially towards gram negative bacteria, and also has anti-tumor activity.

Chemical structure: peptide

Application

Actinomycin D has a wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells, and as an antibiotic in treatment of various malignant neoplasms, including Wilm′s tumour and the sarcomas.

Packaging

2, 5, 10, 25, 100 mg in serum bottle

Biochem/physiol Actions

Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.

Caution

This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.

Preparation Note

Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3462 6.1 / PGII
WGK Germany 
3
RTECS 
AU1575000

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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