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A1410 Sigma

Actinomycin D

from Streptomyces sp., ~98% (HPLC)

Synonym: Actinomycin C1, Actinomycin IV, Dactinomycin

  • CAS Number 50-76-0

  • Empirical Formula (Hill Notation) C62H86N12O16

  • Molecular Weight 1255.42

  •  Beilstein Registry Number 605235

  •  EC Number 200-063-6

  •  MDL number MFCD00005033

  •  PubChem Substance ID 24890541

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Properties

Related Categories A - K, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics A-F,
Quality Level   PREMIUM
biological source   Streptomyces sp.
InChI Key   RJURFGZVJUQBHK-IIXSONLDSA-N
assay   ~98% (HPLC)
form   powder
optical activity   [α]/D alpha: 28/D (Rotation: -315 degrees (c=0.25% in methanol))
color   red to orange
  red, powder
mp   251-253
solubility   DMSO: 1 mg/mL
  ethanol: soluble
Mode of action   DNA synthesis | interferes
antibiotic activity spectrum   neoplastics
storage temp.   2-8°C

Description

Caution

This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.

Packaging

2, 5, 10, 25, 100 mg in serum bottle

Preparation Note

Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.

Application

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Actinomycin D has a wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells, and as an antibiotic in treatment of various malignant neoplasms, including Wilm′s tumour and the sarcomas.

Biochem/physiol Actions

Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.

General description

Actinomycin D is a polypeptide antibiotic first described by Waksman. It is bacteriostatic in nature, especially towards gram negative bacteria, and also has anti-tumor activity.

Chemical structure: peptide

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3462 6.1 / PGII
WGK Germany 
3
RTECS 
AU1575000

Documents

Certificate of Analysis

Certificate of Origin


The Emprove® Program
Protocols & Articles
Peer-Reviewed Papers
15

References

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