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  • A1418 - Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

A1418 Sigma

Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution



Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates,
biological source   goat
recombinant   expressed in goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   mouse
does not react with   human
application(s)   direct ELISA: 1:40,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25
  western blot: 1:50,000-1:100,000 using total cell extract of HeLa cells
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C


Other Notes

Antibody adsorbed with human IgG.


Purified mouse IgG Fc fragment.

Preparation Note

Adsorbed to reduce background with human samples.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide


Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37°C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37°C.

Anti-mouse IgG (Fc specific)–alkaline phosphatase antibody can be used in ELISPOT (immunospot) assay. It can also be used in immunohistochemistry and western blot

General description

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. Anti-mouse IgG (Fc specific)–alkaline phosphatase antibody (diluted 1: 10,000 in PBS-Tween) can be used in ELISA. It is also useful in immunoblotting. Goat anti-mouse IgG (Fc specific)–alkaline phosphatase antibody reacts specifically with mouse IgG Fc fragment, IgG and its subclasses IgG1, IgG2a, IgG2b and IgG3 but not with human IgG, and mouse IgA or IgM.

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NONH for all modes of transport
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