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A1470 Sigma-Aldrich

Bovine Serum Albumin

low endotoxin, lyophilized powder, Cohn Analog, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Properties

Related Categories Albumin, Albumins and Transport Proteins, Albumins by Application, Biochemicals and Reagents, Bovine Serum Albumins,
biological source   bovine
product line   BioReagent
assay   ≥98% (agarose gel electrophoresis)
form   lyophilized powder
mol wt   ~66 kDa
purified by   heat shock fractionation
packaging   poly bottle of
origin   USA origin
application(s)   cell culture | mammalian: suitable
impurities   ≤20 ppm Pb
  ≤3 EU/mg endotoxin
pH   7
solubility   water: soluble (40 mg/ml)
storage temp.   2-8°C

Description

Application

Select the right albumin for your application with the Albumin Selection Guide

Packaging

10, 25, 100 g in poly bottle

5 kg in fiber drum

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Manufactured by a novel process that maintains the lipid and growth factor characteristics of cold-ethanol precipitated albumins.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Legal Information

Cohn Analog is a trademark of Proliant, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Pipette Controller from Brand
Protocols & Articles

Related Content

Albumin in Cell Culture

Importance and uses of albumin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures
Keywords: Cell culture, Culture media, Degradations, Hormones, Ligands, Oxidations, PAGE, Purification, Redox Reactions

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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