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A1543 Sigma-Aldrich

Anti-Human IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates,
conjugate   alkaline phosphatase conjugate
clone   polyclonal
biological source   goat
application(s)   direct ELISA: 1:20,000
  dot blot: 1:30,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
  western blot (chemiluminescent): 1:30,000
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   IgG fraction of antiserum
Quality Level   200
recombinant   expressed in goat
antibody product type   secondary antibodies

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains, with molecular weight of 50kDa and 25kDa respectively. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat anti-human IgG binds to human IgG. Immunoglobulin G (IgG) is part of the immunoglobulin family and is a widely expressed serum antibody. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains.

Immunogen

IgG purified from human serum

Application

Goat anti-human IgG can also be used for dot blot (1:30,000) and immunohistochemical (1:50) applications.

Serum human IgG levels were analyzed by solid phase ELISA assays using alkaline phosphatase-conjugated goat anti-human IgG antibody incubated for 1 hour at 37°C. Assays was developed using 0.1% p-nitrophenyl phosphate (Sigma) as the substrate. Antibodies to the Seoul (strain 80/39) virus was detected in human sera by western blot analysis using alkaline phosphatase-conjugated goat anti-human IgG antibody at a 1:1000 dilution. Blots were developed using nitroblue tetrazolium and 5-bromo-4-chloro-3-indoyl-phosphate substrate (Sigma).

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the source of the alkaline phosphatase that is used to make Product A1543, Anti-Human IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat?
Product A1543, Anti-Human IgG (whole molecule) -Alkaline Phosphatase antibody produced in goat,is prepared using alkaline phosphatase from bovine intestinal mucosa.
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Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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