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A1888 Sigma-Aldrich

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

≥98% (HPLC)

Synonym: AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

  • CAS Number 30931-67-0

  • Empirical Formula (Hill Notation) C18H24N6O6S4

  • Molecular Weight 548.68

  •  Beilstein Registry Number 7329461

  •  EC Number 250-396-6

  •  MDL number MFCD00010404

  •  PubChem Substance ID 24890599

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Description

Application

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).

Packaging

1, 2, 5 g in poly bottle

General description

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA (enzyme-linked immunosorbent assay) procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Other Notes

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
DL7002000
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Protocols

A general protocol for the generation of Nanobodies for structural biology

Els Pardon a, b , Toon Laeremans a, b , Sarah Triest a, b , Søren G F Rasmussen c , Alexandre Wohlkönig a, b , Armin Ruf d , Serge Muyldermans b, e , Wim G J Hol f , Brian K Kobilka g , Jan Steyaert ...
Keywords: AGE, Adsorption, Amplification, Antibiotics, Biochemistry, Biotinylations, Buffers, Cancer, Centrifugation, Chromatography, Cloning, Coagulation, Crystallization, DNA purification, Deaminations, Degradations, Detergents, Digestions, Diseases, Drug discovery, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Genetic, Genomics, Hematology, High performance liquid chromatography, Immobilization, Immunology, Infectious Diseases, Neuroscience, Nucleic acid annealing, Nucleic acid hybridization, Peptide synthesis, Phase transitions, Polymerase chain reaction, Purification, Sample preparations, Separation, Sequencing, Size-exclusion chromatography, Sterilizations, Transfection, Western blot, transformation

Related Content

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (a1888) Discussion and Reviews

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Peer-Reviewed Papers
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References

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