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A1888 Sigma-Aldrich

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

≥98% (HPLC)

Synonym: AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

  • CAS Number 30931-67-0

  • Empirical Formula (Hill Notation) C18H24N6O6S4

  • Molecular Weight 548.68

  •  Beilstein/REAXYS Number 7329461

  •  EC Number 250-396-6

  •  MDL number MFCD00010404

  •  PubChem Substance ID 24890599

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Description

General description

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA (enzyme-linked immunosorbent assay) procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Application

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Packaging

1, 2, 5 g in poly bottle

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
DL7002000

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

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