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A1888 Sigma-Aldrich

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

≥98% (HPLC)

Synonym: AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

  • CAS Number 30931-67-0

  • Empirical Formula (Hill Notation) C18H24N6O6S4

  • Molecular Weight 548.68

  •  Beilstein/REAXYS Number 7329461

  •  EC Number 250-396-6

  •  MDL number MFCD00010404

  •  PubChem Substance ID 24890599

  •  NACRES NA.83

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Properties

Related Categories Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Core Bioreagents, Detection,
assay   ≥98% (HPLC)
form   powder
solubility   water: 10 mg/mL, clear to slightly hazy
storage temp.   2-8°C
SMILES string   [NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
InChI   1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
InChI key   OHDRQQURAXLVGJ-AXMZSLBLSA-N

Description

General description

ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is water soluble chemical compound used as chromogenic substrate:
• ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example).
• ABTS together with laccase or bilirubin oxidase.

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).††† ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.

Application

• 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).
• ABTS is significantly used by the food industry, to measure the antioxidant capacities within different foods.
• ABTS is also used to monitor glucose concentrations in different solutions including blood serum.
• It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results.

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Packaging

1, 2, 5 g in poly bottle

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
DL7002000
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

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