A2058 Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture

Synonym: Albumin bovine serum, BSA, Bovine albumin



Related Categories Albumin, Albumins, Albumins and Transport Proteins, Albumins by Application, Biochemicals and Reagents,
Quality Level   200
biological source   bovine
product line   BioReagent
assay   ≥95% (agarose gel electrophoresis)
form   lyophilized powder
mol wt   ~66 kDa
purified by   chromatography
packaging   poly bottle of
origin   New Zealand origin
application(s)   cell culture | mammalian: suitable
impurities   IgG free
  ≤0.05% IgG
  ≤1 EU/mg endotoxin
pH   7
solubility   water: soluble (40 mg/ml)
storage temp.   2-8°C
Gene Information   bovine ... ALB(280717)


General description

Bovine serum albumin (BSA) is a non-glycosylated protein belonging to the serum albumins family. It is a globular protein with α-helix structure and 17-disulfide bonds. BSA has a molecular weight of 66 kDa consisting three domains and two subdomains under each. BSA shows structural similarity with human serum albumin.


BSA is widely used as a blocking agent in immunoassays and immunodetection procedures, and also as a carrier protein for dilution of antibodies. Thus it is of concern to be aware of potential trace endogenous content of globulins / IgG in BSA, because of concerns that such trace globulins / IgG may serve as antigens for secondary antibodies in immunodetection work. This product undergoes testing for low IgG content / status as essentially immunoglobulin-free.

Select the right albumin for your application with the Albumin Selection Guide

Bovine Serum Albumin has been used to treat polyomavirus BK to serve as a control in testing intravenous immunoglobulins preparations.


1, 5, 25, 100 g in poly bottle

Biochem/physiol Actions

Bovine Serum Albumin (BSA) acts as a carrier protein for drugs, hormones and fatty acids. It is an essential component of cell culture medium. BSA serves as a blocking agent in enzyme linked immunosorbent assay (ELISA) and prevents non-specific binding of antigens and antibodies to the microtiter plates. Due to its high solubility, low cost and purity and interaction with surfactant, it is useful in pharmaceutical and cosmetic industry.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

• Tested for use in cell culture
• Low endotoxin
• Essentially IgG-free (immunoglobulin free)

Preparation Note

Prepared by salt fractionation, ion exchange, and gel filtration chromatography.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cancer, Cell attachment, Cell culture, Cryopreservation, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Related Content

Albumin in Cell Culture

Importance and uses of albumin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures
Keywords: Cell culture, Culture media, Degradations, Hormones, Ligands, Oxidations, PAGE, Purification, Redox Reactions

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?