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  • A2172 - Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse

A2172 Sigma

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse

clone 5C5, ascites fluid



Related Categories AC-AC, Actin Antibodies, Alphabetical Index, Antibodies, Antibodies for Cardiac Stem Cells,
species reactivity   sheep, snake, rabbit, human, rat, guinea pig, frog, bovine, carp
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle
  immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle
  indirect ELISA: suitable
  western blot: suitable
clone   5C5, monoclonal
antibody form   ascites fluid
isotype   IgM
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTA1(58), ACTC1(70)
mouse ... Rwdd2a(69519)
rat ... Acta1(29437), Actc1(29275)
biological source   mouse
conjugate   unconjugated



rabbit striated muscle actin.

General description

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.
Mouse monoclonal anti-actin (α-sarcomeric) antibody is specific for α-skeletal and α-cardiac muscle actins as characterized by ELISA, immunoblot and immunohistochemistry. The antibody shows wide species cross reactivity with tissue from human, sheep, bovine, rabbit, guinea pig, rat, frog, snake, and carp. However, it does not react with smooth muscle tissue.

Physical form

The product is provided as ascites fluid containing 0.1% sodium azide as a preservative.


Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Mouse monoclonal anti-actin (alpha-sarcomeric) antibody has been used for western blot, and immunohistochemistry applications. The antibody can also be used for indirect ELISA.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibodies to Actin Gene Family Isoforms

The Actin Gene Family Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular p...
Keywords: Western blot

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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