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A2172 Sigma-Aldrich

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse

clone 5C5, ascites fluid

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Properties

Related Categories AC-AC, Actin Antibodies, Alphabetical Index, Antibodies, Antibodies for Cardiac Stem Cells,
conjugate   unconjugated
clone   5C5, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle
  immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle
  indirect ELISA: suitable
  western blot: suitable
species reactivity   carp, rat, bovine, sheep, snake, frog, guinea pig, human, rabbit
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgM
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P68032
  P68133
Gene Information   human ... ACTA1(58), ACTC1(70)
mouse ... Rwdd2a(69519)
rat ... Acta1(29437), Actc1(29275)

Description

General description

Monoclonal Anti-α-Sarcomeric Actin (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin is one of the most conserved eukaryotic proteins and the isoforms show >90% overall sequence homology. It is expressed in mammals and birds as six isoforms. Four of the six represent differentiation markers of muscle tissues. The other two are found in practically all cells.

Immunogen

rabbit striated muscle actin.

Application

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse has also been used
• immunofluorescence
• double immunostaining
• double labelling terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) immunofluorescence staining.

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Mouse monoclonal anti-actin (alpha-sarcomeric) antibody has been used for western blot, and immunohistochemistry applications.

Physical form

The product is provided as ascites fluid containing 0.1% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibodies to Actin Gene Family Isoforms

The Actin Gene Family Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular p...
Keywords: Western blot

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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