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A2189 Sigma-Aldrich

Monoclonal Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in mouse

clone MB-11, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, HU-HZ, Human IgM Secondary Antibodies and Conjugates,
conjugate   alkaline phosphatase conjugate
clone   MB-11, monoclonal
biological source   mouse
application(s)   direct ELISA: 1:35,000
  dot blot: 1:20,000 using 20 ng of human IgM/dot
  dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence)
species reactivity   human
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG2b
Quality Level   200
antibody product type   secondary antibodies

Description

General description

Human IgMs are the initial immunoglobulin isotypes that appear in blood in response to the first exposure to external antigens. IgMs have been implicated in CNS myelin repair and increased secretory IgM levels have been linked to rhino-conjunctivitis . Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM. The product does not react with human IgG, IgA or light chains.

Monoclonal Anti-Human IgM (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Human IgM isolated from pooled normal human serum

Application

Monoclonal Anti-Human IgM (μ-chain specific)?Alkaline Phosphatase antibody produced in mouse has been used in immunocytochemistry and enzyme linked immunosorbent assay (ELISA).

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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