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  • A2229 - Anti-Apolipoprotein J antibody produced in goat

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Properties

Related Categories AO-AQ, Alphabetical Index, Antibodies, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   ELISA: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200
  western blot: 1:5,000-1:10,000
species reactivity   human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   2-8°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P10909
Gene Information   human ... CLU(1191)

Description

General description

Apolipoprotein J is a ubiquitously expressed glycoprotein that is involved in the differentiation and proliferation of cells. Apolipoprotein J in conjunction with apolipoprotein E may affect the onset of amyloid-beta deposition, and also may be involved in the local toxicity associated with these deposits. Hence, apolipoprotein J may function as a cerebrospinal fluid marker for Alzheimer′s disease.
Goat anti-apolipoprotein J antibody recognizes human apolipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apolipoproteins.

Immunogen

Apolipoprotein Type J isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Application

Goat anti-apolipoprotein J antibody can be used for immunohistochemistry applications using formalin-fixed, paraffin-embedded sections at a concentration ranging from 1:50-1:200. The antibody is also suitable for use in ELISA.

HEK293 cells were transfected with either an expression vector to over expression Clusterin or siRNA to knock down expression of Clusterin. Clusterin levels were monitored by western blot using goat anti-Clusterin antibody at 1:1000.

Physical form

Solution in 0.1 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, and 0.01% sodium azide as preservative.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

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