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A2441 Sigma

Anti-Axl antibody produced in goat

affinity isolated antibody, lyophilized powder



Related Categories ATH-AZ, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
species reactivity   mouse, human (10%)
application(s)   indirect ELISA: 0.5 μg/mL using 0.12 ng/well Axl (ELISA - 0.5 μg/ml detects 0.12 ng/well Axl.)
  western blot: suitable (0.1-0.2 μg/ml detects Axl 2 ng/lane.)
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
mol wt   antigen mol wt 100-110 kDa
storage temp.   −20°C
Gene Information   human ... AXL(558)
mouse ... Axl(26362)
biological source   goat
conjugate   unconjugated



recombinant mouse Axl, a tyrosine kinase receptor.

General description

Axl (Ark or Ufo) belongs to the TAM family of receptor tyrosine kinases; the other two members of the family are Tyro-s (Sky) and Mer. Axl was detected in patients with chronic myelogenous leukemia. It is ubiquitously expressed in a variety of cells including those of brain, blood (monocytes, macrophages, platelets), liver, kidney, testes, heart and skeletal muscle. The ligands for Axl are vitamin-K-dependent proteins, Gas 6 and protein S. Signaling mediated by Axl when activated by Gas 6 regualte cell survival, proliferation, adhesion and migration. Overexpression of Axl has been reported in human cancers of colon, lung, breast, astrocytes, thyroid, esophagus and liver.
Anti-Axl antibody specifically recognizes mouse Axl (~73.8 kDa). It shows 10% cross-reactivity with human Axl.

The reduced mouse Axl/Fc monomer has a calculated molecular mass of approx. 73.8kDa. Due to glycosylation, the recombinant protein migrates as a 100 -110kDa protein in SDS-PAGE under reducing conditions.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (pH 7.4) with 5% trehalose.


A working antibody concentration of 0.1-0.2 μg/mL may be used to detect mouse Axl by immunoblotting. The antibody may be used in indirect ELISA wherein antibody concentration of 0.5-1.0 μg/mL detects Axl of ~ 0.12 ng/well.

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