• USA Home
  • A25120 - DEAE–Sephadex® A-25 chloride form

A25120 Sigma-Aldrich

DEAE–Sephadex® A-25 chloride form

Synonym: DEAE Sephadex®, Diethylaminoethyl Sephadex®



Related Categories Analytical/Chromatography, Anion Exchange Media - Ion Exchange Chromatography , Ion Exchange Chromatography, Molecular Biology, Protein Chromatography,
Quality Level   200
bead size   40-125 μm (dry)
pore size   ~30,000 Da exclusion limit
operating pH   2 - 9
capacity   3-4 meq/g ion exchange capacity
compatibility   mode of use mixed weak and strong anion exchange chromatography


General description

A25120-100G′s update product number is GE17-0170-01
A25120-500G′s update product number is GE17-0180-02


DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins. It has been used to identify and label an insulin activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients.


10, 50, 100, 500 g in poly bottle

Legal Information

DEAE Sephadex is a registered trademark of Cytiva

Sephadex is a registered trademark of Cytiva

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How should I store Product A25120, DEAE-Sephadex® A-25 chloride?
Unused media can be stored in the container at +4 to +30 °C.Ensure that the screw-top is fully tightened.Packed columns should be equilibrated in working buffer containing 20% ethanol to prevent microbial growth.
What is the chemical stability of Product A25120, DEAE-Sephadex® A-25 chloride form?
According to the manufacturer, this is compatible with all commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride and 70% ethanol.
How do I equilibrate Product A25120, DEAE-Sephadex® A-25 chloride form?
After packing, and before a chromatographic run, equilibrate with working buffer by washing with at least 5 bed volumes.
How should I regenerate Product A25120, DEAE-Sephadex® A-25 chloride form?
After each separation, elute any reversibly bound material either with a high ionic strength solution (e.g. 1 M NaCl in buffer) or by increasing pH. Regenerate the media by washing with at least 5 bed volumes of buffer, or until the column effluent shows stable conductivity and pH values.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles


Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Combinatorial synthesis, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Ion exchange resins, Pharmaceutical, Purification, Separation

Peer-Reviewed Papers


Related Products

replaced by

Product #



Add to Cart

GE17-0180-02 DEAE-Sephadex® A-50, Cytiva, 17-0180-02, pack of 500 g same as A25120-500G
GE17-0170-01 DEAE-Sephadex® A-25, Cytiva, 17-0170-01, pack of 100 g same as A25120-100G

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?