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A269 Sigma-Aldrich

Anti-A2A Adenosine Receptor antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories AD-AD, Alphabetical Index, Antibodies, Antibodies for Neurotransmission, Antibodies to Neurotransmitter Receptors,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (frozen sections): 1:100
  immunoprecipitation (IP): suitable
  western blot: 1:2000
species reactivity   human, canine
should not react with   rat
mol wt   antigen mol wt ~45 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P29274
Gene Information   human ... ADORA2A(135)

Description

General description

ADORA2A is an adenosine receptor and part of the G-protein coupled receptor family of proteins. ADORA2A is involved in regulating myocardial blood flow and dopamine release in the brain.

Anti-A2A Adenosine Receptor is specific for A2A adenosine receptor adenosine receptor subtype. By immunoblotting, it reacts strongly with canine A2A. However, it does not react with rat A2A. The antibody detects A2A adenosine receptor in human hippocampus by immunohistology and may be used for immunoprecipitation.

Immunogen

Synthetic peptide (Ser-His-Gly-Asp-Met-Gly-Leu-Pro-Asp-Val-Glu-Leu-Leu-Ser-His-Glu-Leu-Lys) derived from amino acids 373-391 of the canine A2A adenosine receptor C-terminal domain.

Application

Rabbit anti-A2A adenosine receptor antibody can be used for western blot applications. The antibody can also be used for immunohistochemistry (1:100, frozen sections) and immunoprecipitation applications.

Physical form

Solution in phosphate buffered saline, containing 1 mg/ml BSA and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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