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  • A271 - Anti-α2A Adrenergic Receptor antibody produced in rabbit

A271 Sigma

Anti-α2A Adrenergic Receptor antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution



Related Categories AD-AD, Alphabetical Index, Antibodies, Antibodies for Neurotransmission, Antibodies to Neurotransmitter Receptors,
species reactivity   mouse, pig, human, rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000
  western blot: 1:500
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~45 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ADRA2A(150)
mouse ... Adra2a(11551)
rat ... Adra2a(25083)
biological source   rabbit
conjugate   unconjugated



synthetic peptide derived from amino acids 218-235 of human, mouse, rat, and pig α2A adrenergic receptor. The immunogen sequence is found within the third intracellular loop of the α2A adrenergic receptor subtype.

General description

Adrenergic receptors (ARs) belong to the 7-transmembrane domain G protein-coupled receptor superfamily that binds to the endogenous catecholamines, epinephrine and norepinephrine. α2AR subtypes block adenylyl cyclase, suppress voltage-sensitive Ca2+ channels, and stimulate receptor dependent K+ channels. All of the α2AR subtypes inhibit adenylyl cyclase through coupling to members of the Gi/Go class of G proteins. They are an important component of the neural circuitry and modulate cardiovascular physiology. The physiological function of the α2ARs in the kidney is to regulate Na+/H+ exchange.
Anti-α2A adrenergic receptor detects rat and mouse α2A adrenergic receptors by immunoblotting and immunohistochemistry. In immunoblotting, the antibody detects an approx. 45kDa band representing α2A receptors in tissues expressing this receptor subtype, although some non-specific bands have also been observed in certain cases.

Physical form

Solution in phosphate buffered saline, containing 1 mg/ml BSA and 0.05% sodium azide.


Rabbit anti-α2A adrenergic receptor antibody can be used for western blot (1:500) and immunohistochemistry (1:1000) applications.

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Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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