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A2765 Sigma-Aldrich

Azocasein

protease substrate

Synonym: Sulfanilamide-azocasein

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Properties

Related Categories Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience, Enzyme Substrates, Enzymes, Inhibitors, and Substrates,
Quality Level   200
ε (extinction coefficient)   ≥25 at 440 nm in 0.1 M NaOH at 1%
storage temp.   2-8°C

Description

General description

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Application

Azocasein has been used as a substrate for determination of protease activity.

Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.

Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.

Packaging

1, 5, 10, 25 g in glass bottle

Linkage

View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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