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A2765 Sigma-Aldrich

Azocasein

protease substrate

Synonym: Sulfanilamide-azocasein

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Description

Linkage

View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer

Packaging

1, 5, 10, 25 g in glass bottle

Application

Azocasein has been used as a substrate for determination of protease activity.

Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.

Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.

General description

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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