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  • A277 - Monoclonal Anti-Na+/K+ ATPase (α1 Subunit) antibody produced in mouse

A277 Sigma

Monoclonal Anti-Na+/K+ ATPase (α1 Subunit) antibody produced in mouse

clone M8-P1-A3, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Ion Channels, Antibodies to Ion Pumps,
species reactivity   sheep, pig, canine, rat
does not react with   Xenopus, chicken
application(s)   immunohistochemistry (frozen sections): 1:100
  western blot: 1:250
clone   M8-P1-A3, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt ~110 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Atp1a1(24211)
biological source   mouse
conjugate   unconjugated



lamb kidney α1 subunit of Na+/K+ ATPase.

General description

Epitope mapping studies indicates the epitope is between amino acids 496 and 506 of lamb kidney Na+/K+ ATPase. No reactivity is observed in chicken or Xenopus. Staining is consistent with the plasma membrane localization of Na+/K+ ATPase.

Mouse monoclonal anti-Na+/K+ ATPase (α1 subunit) reacts with the α1 subunit of Na+/K+-ATPase in sheep, canine, rat and pig. In rat, the antibody shows no reactivity with the α2 or α3 subunits.

Na+/K+ transporting ATPase subunit α 1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.


Mouse monoclonal anti-Na+/K+ ATPase (α1 subunit) has been used for western blot and co-immunoprecipitation assays. The product can also be used for immunohistochemistry applications using frozen sections at a dilution of 1:100.

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Antibody Basics

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Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
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