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  • A278 - Monoclonal Anti-Na+/K+ ATPase (β1 Subunit) antibody produced in mouse

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A278 Sigma-Aldrich

Monoclonal Anti-Na+/K+ ATPase (β1 Subunit) antibody produced in mouse

clone M17-P5-F11

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Ion Channels, Antibodies to Ion Pumps,
conjugate   unconjugated
clone   M17-P5-F11, monoclonal
application(s)   immunohistochemistry (frozen sections): 1:100
  western blot: 1:1,000-1:10,000
species reactivity   pig, human, sheep, canine
should not react with   rat
mol wt   antigen (kidney) 55 kDa
  antigen (liver) 42 kDa
shipped in   dry ice
storage temp.   −20°C
isotype   IgG2a
antibody product type   primary antibodies
UniProt accession no.   P05026
Gene Information   human ... ATP1B1(481)

Description

General description

Epitope mapping studies indicate that this clone recognizes the epitope between amino acids 195 and 199 of the sheep β1 subunit of Na+/K+ ATPase. Staining is consistent with the plasma membrane localization of Na+/K+ ATPase.

Mouse monoclonal anti-Na+/K+ ATPase (β1 subunit) reacts with the β1 subunit of Na+/K+ ATPase in humans, sheep, canines and pigs, but not in rats. The product shows no reactivity with rat β2 subunit. In immunoblotting, the antibody detects the β1 subunit of Na+/K+ ATPase as a 42kDa band in liver samples, and as a 52kDa band in kidney samples.

Na+/K+ transporting ATPase subunit α1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.

Immunogen

lamb kidney β subunit of Na+/K+ ATPase.

Application

Mouse Monoclonal Anti-Na+/K+ ATPase (β1 subunit) can be used in immunohistochemical (1:100) and western blot (1:1,000 - 1:10,000) applications

Physical form

Solution in phosphate buffered saline with 1mg/ml BSA and 0.05% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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